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Isolation, molecular identification and genomic pattern of Mycobacterium bovis isolates collected from tuberculin-positive cattle in infected farms of Shiraz

Nader Mosavari

Mycobacterium bovis is the main cause of tuberculosis in cattle. At global scale and also in Iran, the most frequent currently-in-use method in detection of infected cattle is tuberculination.The present study was aimed to improve our genomic knowledge of Mycobacterium bovis population structure in cattle farms of Shiraz. Fifty pathological samples from tuberculin-positive cattle collected from two slaughterhouses at Shiraz were subjected to bacterial culture on glycerinated and pyruvated Lowenstein-Jensen media. Genomic material from culture-positive slopes was extracted and used in PCR-16S rRNA, PCR-IS6110, and PCR-RD typing experiments.