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Evaluation of diagnostic potential of recombinant D-erythrulose 1-phosphate dehydrogenase using indirect enzyme-linked immunosorbent assay for diagnosis of bovine brucellosis.

W S A A Shell

Serological tests used for diagnosis of bovine brucellosis are usually depending on smooth lipopolysaccharides (S-LPS) as a diagnostic antigen which usually gives false positive reactions. So, our study aims to produce and evaluate a diagnostic kit for accurate diagnosis of bovine brucellosis differentiating between vaccinated and infected cattle and exclusion of false positive cases. Idea of this kit depends upon the fact that The EryC gene is absent in Brucella abortus S19 only but it is present and functional in all other Brucella strains and isolates so according to these facts, the use of ELISA kit coated with single subunit (recombinant) EryC protein may be useful, rather than S-LPS, as an alternative diagnostic antigen in diagnosis of bovine brucellosis and differentiation between S19 vaccinated and Brucella infected cattle.