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Dot-Enzyme Linked Immunosorbent Assay for Diagnosis of Ovine Oesophagostomosis

Rajeshwari Gaddam, Murthy GSS, Sudhakar Kommu,

Dot Enzyme Linked Immunosorbent Assay (Dot-ELISA) was standardized and evaluated to detect anti-Oesophagostomum antibodies in sera of sheep by using three different antigenic components obtained from the parasite Oesophagostomum i.e., Crude Somatic Antigen (CSA), Excretory Secretory Antigen (ESA) and Larval Antigen (LA). Dot ELISA was standardized at an antigenic concentrations of 0.06 μg CSA, 0.3 μg of ESA and 0.1 μg of LA with sera at 1:100 dilution with Phosphate buffered saline with Tween-20 (0.1% PBST), using sheep anti-rabbit antibodies with horse radish peroxidase enzyme as conjugate at 1:5000 concentration and di-amino benzedine as substrate. The sensitivity, specificity of test were 73.33%, 66.67% by using CSA; 80%, 72.22% by using ESA and as 40%, 66.67% by using LA with statistically significant p value (p<0.05). By using this test 22.01, 10.02, 5.5 were percentage positives detected respective to their antigens, crude somatic antigen, excretory secretary antigen and larval antigen.