抽象的

Chicken immunologically active proteins for the development of anti-idiotypic vaccines

Ioana Manea

 Introduction: According to Jerne???s network theory, antibodies contain in their variable region a representation of the ???universe??? of antigenic structures, the idiotype. It is possible to induce antibodies against the antigen-binding site of other antibodies (2-6); these new antibodies, called anti-idiotypic (Ab2B), can be used to manipulate the immune system (1,5,6). They have been successfully used in the induction of humoral immune responses against several antigens including bacteria and viruses (2,3,5,6). Chicken Immunologically Active Proteins [CIAP] including Immunoglobulin (Ig)-Y, that are produced by immunizing chickens, have further advantages compared with mammalian IgG (1,2,5,6). This study investigates the use of the chicken and egg system for the development of an immune response against antimicrobial resistance (AMR) bacteria. Methodology: Stage I: Brown leghorn chickens were immunized with I-spga immunogen which contained antigens from more than 20 inactivated AMR bacteria. Indirect ELISA was used to measure anti-bacterial antibody titers in the watery soluble fraction of eggs up to 14 weeks after the third immunization. Stage II: chicken groups have been formed that have individually consumed yolk or white egg from either hiperimune eggs or from eggs produced by unimmunized chicken. At the end of the experiment, presence of antibodies against original AMR bacteria was checked by ELISA in blood samples and eggs of birds used in the Stage II of the experiment. Results: Antibodies against AMR bacteria were detected only in the blood and eggs of chicken that consumed hiperimmune eggs; these antibodies inhibited the growth of AMR bacteria in vitro.