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ADVERSE EFFECTS OF DELTAMETHRIN ON THE CEREBELLUM OF MOTHERS RATS AND THEIR OFFSPRING AND THE POSSIBLE AMELIORATIVE ROLE OF MELATONIN

Abd El-Fattah BM El-Beltagy, Kadry AM Elbakry, Mohamed MA Elghazaly, Lashin S Ali and Norhan HM EL Daqaqq

Deltamethrin is the most famous neurotoxic synthetic pyrethroid. The present work was mainly designed to evaluate the possible ameliorative role of melatonin against deltamethrin induced cerebellar neurotoxicity in pregnant rats and their offspring. In the present work, 24 pregnant Albino rats were divided into four groups (n=6). The control group received 1 ml/kg bw of ethanol (99%), the melatonin group received a daily oral dose of melatonin (10 mg/kg bw), deltamethrin treated group was received 12.5 mg/kg bw daily of deltamethrin and ameliorative group received deltamethrin simultaneously with melatonin. All groups were received their applicable dose from the 4th day of gestation till the end of weaning period (21th day). The result of the present work revealed that deltamethrin can induce severe histopathological changes in the cerebellar cortex of pregnant rats and their offspring. The immunohistochemical results elucidated strong positive expression for GFAP and weak to moderate immune reaction for synaptophysin in the cerebellar cortex of deltamethrin treated mother's rats and their offspring. Moreover, in deltamethrin treated rats, the serum levels of antioxidants enzymes (SOD, CAT and GSH) as well as serotonin and glutamate showed highly significant decrease if compared with control. Cerebellar tissue analysis revealed that deltamethrin is implicated in induction of apoptosis that confirmed by increased activity of Caspse-3 and decreased activity of bcl2. Post supplementation of melatonin to deltamethrin, successfully ameliorated the adverse histopathological, immmunohistochemical and biochemical changes in cerebellum of mother's rats and their offspring.